Lab techniques

From Dougster BioWiki
Revision as of 07:10, 12 November 2020 by Irina (talk | contribs)
Jump to navigation Jump to search




Chromatography= physical method of separation that distributes components to separate between two phases. The two phases are: Stationary phase, and mobile phase.

paper chromatography= uses paper as the stationary phase, and a solvent as the mobile phase. Solvent can be water. The separation method is based on polarity.

Cell fractionation and centrifugation: Procedure that splits apart a cell by adding a chemical. Then you spin it to separate out different parts according to the differences in size.

Resolution:

Electron microscope: Shoots beam of electrons to see small objects.

Light microscope: Uses several lenses to see small objects.

Molecular mass= the molecular mass of a molecule is equal to the sum of the atomic masses of it's elements.

Avogadro's number= 6.02*10^23

Molar mass= a molecule's molar mass is equal to the molecule's molecular mass number in grams. This is called 1 mole. It is also equal in mass to an Avogadro's number amount of the molecule.

Organic chemistry = Study of compounds containing carbon.

Gel electrophoresis = A technique used to separate DNA fragments and other macromolecules by size and charge. You get a gel put samples on it, and then electrify the gel. The smaller DNA fragments will move to the positive side faster than the larger ones.