Difference between revisions of "Lab techniques"
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Cell fractionation and centrifugation: Procedure that splits apart a cell by adding a chemical. Then you spin it to separate out different parts according to the differences in size. | Cell fractionation and centrifugation: Procedure that splits apart a cell by adding a chemical. Then you spin it to separate out different parts according to the differences in size. | ||
| − | Resolution: | + | Resolution: '''DEFINITION MISSING''' |
Electron microscope: Shoots beam of electrons to see small objects. | Electron microscope: Shoots beam of electrons to see small objects. | ||
| − | Light microscope: Uses several lenses to see small objects. | + | Light microscope: Uses several lenses to see small objects. '''SMALL OBJECTS NOT SPECIFIC ENOUGH...PLEASE INCLUDE WHEN IT IS APPROPRIATE TO USE THEM (EX: LIVING CELLS, MAX MAGNIFYING POWER)....THERE WAS AN IMAGE IN TEXTBOOK THAT WOULD BE USEFUL TO INCLUDE.''' |
Molecular mass= the molecular mass of a molecule is equal to the sum of the atomic masses of it's elements. | Molecular mass= the molecular mass of a molecule is equal to the sum of the atomic masses of it's elements. | ||
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Gel electrophoresis = A technique used to separate DNA fragments and other macromolecules by size and charge. You get a gel put samples on it, and then electrify the gel. The smaller DNA fragments will move to the positive side faster than the larger ones. | Gel electrophoresis = A technique used to separate DNA fragments and other macromolecules by size and charge. You get a gel put samples on it, and then electrify the gel. The smaller DNA fragments will move to the positive side faster than the larger ones. | ||
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| + | '''SPECTROMETRY (MEASURING ABSORPTION AND TRANSMITTANCE OF LIGHT THROUGH A SAMPLE)''' | ||
Revision as of 10:49, 22 December 2020
Chromatography= physical method of separation that distributes components to separate between two phases. The two phases are: Stationary phase, and mobile phase.
paper chromatography= uses paper as the stationary phase, and a solvent as the mobile phase. Solvent can be water. The separation method is based on polarity.
Cell fractionation and centrifugation: Procedure that splits apart a cell by adding a chemical. Then you spin it to separate out different parts according to the differences in size.
Resolution: DEFINITION MISSING
Electron microscope: Shoots beam of electrons to see small objects.
Light microscope: Uses several lenses to see small objects. SMALL OBJECTS NOT SPECIFIC ENOUGH...PLEASE INCLUDE WHEN IT IS APPROPRIATE TO USE THEM (EX: LIVING CELLS, MAX MAGNIFYING POWER)....THERE WAS AN IMAGE IN TEXTBOOK THAT WOULD BE USEFUL TO INCLUDE.
Molecular mass= the molecular mass of a molecule is equal to the sum of the atomic masses of it's elements.
Avogadro's number= 6.02*10^23
Molar mass= a molecule's molar mass is equal to the molecule's molecular mass number in grams. This is called 1 mole. It is also equal in mass to an Avogadro's number amount of the molecule.
Organic chemistry = Study of compounds containing carbon.
Gel electrophoresis = A technique used to separate DNA fragments and other macromolecules by size and charge. You get a gel put samples on it, and then electrify the gel. The smaller DNA fragments will move to the positive side faster than the larger ones.
SPECTROMETRY (MEASURING ABSORPTION AND TRANSMITTANCE OF LIGHT THROUGH A SAMPLE)