Difference between revisions of "Lab techniques"
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Cell fractionation and centrifugation: Procedure that splits apart a cell by adding a chemical. Then you spin it to separate out different parts according to the differences in size. | Cell fractionation and centrifugation: Procedure that splits apart a cell by adding a chemical. Then you spin it to separate out different parts according to the differences in size. | ||
| − | Resolution: | + | Resolution: Resolution is how much detail a microscope can see at a certain zoom. |
| − | Electron microscope: Shoots beam of electrons to see small objects. | + | Electron microscope: Shoots beam of electrons to see very small objects. |
| − | Light microscope: Uses several lenses to see | + | Light microscope: Uses several lenses to see living cells and other similar sized objects. |
Molecular mass= the molecular mass of a molecule is equal to the sum of the atomic masses of it's elements. | Molecular mass= the molecular mass of a molecule is equal to the sum of the atomic masses of it's elements. | ||
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Gel electrophoresis = A technique used to separate DNA fragments and other macromolecules by size and charge. You get a gel put samples on it, and then electrify the gel. The smaller DNA fragments will move to the positive side faster than the larger ones. | Gel electrophoresis = A technique used to separate DNA fragments and other macromolecules by size and charge. You get a gel put samples on it, and then electrify the gel. The smaller DNA fragments will move to the positive side faster than the larger ones. | ||
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| + | SPECTROMETRY: MEASURING ABSORPTION AND TRANSMITTANCE OF LIGHT THROUGH A SAMPLE. | ||
Latest revision as of 07:09, 4 January 2021
Chromatography= physical method of separation that distributes components to separate between two phases. The two phases are: Stationary phase, and mobile phase.
paper chromatography= uses paper as the stationary phase, and a solvent as the mobile phase. Solvent can be water. The separation method is based on polarity.
Cell fractionation and centrifugation: Procedure that splits apart a cell by adding a chemical. Then you spin it to separate out different parts according to the differences in size.
Resolution: Resolution is how much detail a microscope can see at a certain zoom.
Electron microscope: Shoots beam of electrons to see very small objects.
Light microscope: Uses several lenses to see living cells and other similar sized objects.
Molecular mass= the molecular mass of a molecule is equal to the sum of the atomic masses of it's elements.
Avogadro's number= 6.02*10^23
Molar mass= a molecule's molar mass is equal to the molecule's molecular mass number in grams. This is called 1 mole. It is also equal in mass to an Avogadro's number amount of the molecule.
Organic chemistry = Study of compounds containing carbon.
Gel electrophoresis = A technique used to separate DNA fragments and other macromolecules by size and charge. You get a gel put samples on it, and then electrify the gel. The smaller DNA fragments will move to the positive side faster than the larger ones.
SPECTROMETRY: MEASURING ABSORPTION AND TRANSMITTANCE OF LIGHT THROUGH A SAMPLE.