Difference between revisions of "Lab techniques"
| Line 9: | Line 9: | ||
paper chromatography= uses paper as the stationary phase, and a solvent as the mobile phase. Solvent can be water. The separation method is based on polarity. | paper chromatography= uses paper as the stationary phase, and a solvent as the mobile phase. Solvent can be water. The separation method is based on polarity. | ||
| − | Cell fractionation: | + | Cell fractionation and centrifugation: Procedure that splits apart a cell by adding a chemical. Then you spin it to separate out different parts according to the differences in size. |
| + | |||
| + | Resolution: | ||
Electron microscope: Shoots beam of electrons to see small objects. | Electron microscope: Shoots beam of electrons to see small objects. | ||
Revision as of 08:09, 9 November 2020
Chromatography= physical method of separation that distributes components to separate between two phases. The two phases are: Stationary phase, and mobile phase.
paper chromatography= uses paper as the stationary phase, and a solvent as the mobile phase. Solvent can be water. The separation method is based on polarity.
Cell fractionation and centrifugation: Procedure that splits apart a cell by adding a chemical. Then you spin it to separate out different parts according to the differences in size.
Resolution:
Electron microscope: Shoots beam of electrons to see small objects.
Light microscope: Uses several lenses to see small objects.
Molecular mass= the molecular mass of a molecule is equal to the sum of the atomic masses of it's elements.
Avogadro's number= 6.02*10^23
Molar mass= a molecule's molar mass is equal to the molecule's molecular mass number in grams. This is called 1 mole. It is also equal in mass to an Avogadro's number amount of the molecule.
Organic chemistry = Study of compounds containing carbon.